This page was generated on 2020-10-17 11:55:03 -0400 (Sat, 17 Oct 2020).
R version 4.0.3 (2020-10-10) -- "Bunny-Wunnies Freak Out"
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> library(testthat)
> library(Maaslin2)
>
> test_check("Maaslin2")
[1] "Creating output folder"
[1] "Creating output figures folder"
2020-10-17 02:46:29 INFO::Writing function arguments to log file
2020-10-17 02:46:29 INFO::Verifying options selected are valid
2020-10-17 02:46:29 INFO::Determining format of input files
2020-10-17 02:46:29 INFO::Input format is data samples as rows and metadata samples as rows
2020-10-17 02:46:29 INFO::Formula for random effects: expr ~ (1 | site) + (1 | subject)
2020-10-17 02:46:29 INFO::Formula for fixed effects: expr ~ diagnosis + dysbiosisnonIBD + dysbiosisUC + dysbiosisCD + antibiotics + age
2020-10-17 02:46:29 INFO::Running selected normalization method: TSS
2020-10-17 02:46:30 INFO::Filter data based on min abundance and min prevalence
2020-10-17 02:46:30 INFO::Total samples in data: 1595
2020-10-17 02:46:30 INFO::Min samples required with min abundance for a feature not to be filtered: 159.500000
2020-10-17 02:46:30 INFO::Total filtered features: 0
2020-10-17 02:46:30 INFO::Filtered feature names:
2020-10-17 02:46:30 INFO::Bypass z-score application to metadata
2020-10-17 02:46:30 INFO::Running selected transform method: AST
2020-10-17 02:46:30 INFO::Running selected analysis method: LM
2020-10-17 02:46:30 INFO::Fitting model to feature number 1, Bifidobacterium.adolescentis
2020-10-17 02:46:31 INFO::Fitting model to feature number 2, Bifidobacterium.bifidum
2020-10-17 02:46:31 INFO::Fitting model to feature number 3, Bifidobacterium.longum
2020-10-17 02:46:32 INFO::Fitting model to feature number 4, Bifidobacterium.pseudocatenulatum
2020-10-17 02:46:32 INFO::Fitting model to feature number 5, Collinsella.aerofaciens
2020-10-17 02:46:32 INFO::Fitting model to feature number 6, Bacteroides.caccae
2020-10-17 02:46:32 INFO::Fitting model to feature number 7, Bacteroides.cellulosilyticus
2020-10-17 02:46:32 INFO::Fitting model to feature number 8, Bacteroides.dorei
2020-10-17 02:46:33 INFO::Fitting model to feature number 9, Bacteroides.eggerthii
2020-10-17 02:46:33 INFO::Fitting model to feature number 10, Bacteroides.faecis
2020-10-17 02:46:33 INFO::Fitting model to feature number 11, Bacteroides.finegoldii
2020-10-17 02:46:34 INFO::Fitting model to feature number 12, Bacteroides.fragilis
2020-10-17 02:46:34 INFO::Fitting model to feature number 13, Bacteroides.intestinalis
2020-10-17 02:46:34 INFO::Fitting model to feature number 14, Bacteroides.massiliensis
2020-10-17 02:46:34 INFO::Fitting model to feature number 15, Bacteroides.ovatus
2020-10-17 02:46:35 INFO::Fitting model to feature number 16, Bacteroides.salyersiae
2020-10-17 02:46:35 INFO::Fitting model to feature number 17, Bacteroides.stercoris
2020-10-17 02:46:35 INFO::Fitting model to feature number 18, Bacteroides.thetaiotaomicron
2020-10-17 02:46:36 INFO::Fitting model to feature number 19, Bacteroides.uniformis
2020-10-17 02:46:36 INFO::Fitting model to feature number 20, Bacteroides.vulgatus
2020-10-17 02:46:36 INFO::Fitting model to feature number 21, Bacteroides.xylanisolvens
2020-10-17 02:46:36 INFO::Fitting model to feature number 22, Bacteroidales.bacterium.ph8
2020-10-17 02:46:37 INFO::Fitting model to feature number 23, Barnesiella.intestinihominis
2020-10-17 02:46:37 INFO::Fitting model to feature number 24, Coprobacter.fastidiosus
2020-10-17 02:46:37 INFO::Fitting model to feature number 25, Odoribacter.splanchnicus
2020-10-17 02:46:38 INFO::Fitting model to feature number 26, Parabacteroides.distasonis
2020-10-17 02:46:38 INFO::Fitting model to feature number 27, Parabacteroides.goldsteinii
2020-10-17 02:46:38 INFO::Fitting model to feature number 28, Parabacteroides.merdae
2020-10-17 02:46:38 INFO::Fitting model to feature number 29, Parabacteroides.unclassified
2020-10-17 02:46:39 INFO::Fitting model to feature number 30, Paraprevotella.clara
2020-10-17 02:46:39 INFO::Fitting model to feature number 31, Paraprevotella.unclassified
2020-10-17 02:46:39 INFO::Fitting model to feature number 32, Prevotella.copri
2020-10-17 02:46:40 INFO::Fitting model to feature number 33, Alistipes.finegoldii
2020-10-17 02:46:40 INFO::Fitting model to feature number 34, Alistipes.onderdonkii
2020-10-17 02:46:40 INFO::Fitting model to feature number 35, Alistipes.putredinis
2020-10-17 02:46:40 INFO::Fitting model to feature number 36, Alistipes.shahii
2020-10-17 02:46:41 INFO::Fitting model to feature number 37, Alistipes.unclassified
2020-10-17 02:46:41 INFO::Fitting model to feature number 38, Streptococcus.salivarius
2020-10-17 02:46:41 INFO::Fitting model to feature number 39, Clostridium.bolteae
2020-10-17 02:46:41 INFO::Fitting model to feature number 40, Clostridium.citroniae
2020-10-17 02:46:42 INFO::Fitting model to feature number 41, Clostridium.clostridioforme
2020-10-17 02:46:42 INFO::Fitting model to feature number 42, Clostridium.hathewayi
2020-10-17 02:46:42 INFO::Fitting model to feature number 43, Clostridium.leptum
2020-10-17 02:46:42 INFO::Fitting model to feature number 44, Clostridium.nexile
2020-10-17 02:46:43 INFO::Fitting model to feature number 45, Clostridium.symbiosum
2020-10-17 02:46:43 INFO::Fitting model to feature number 46, Flavonifractor.plautii
2020-10-17 02:46:43 INFO::Fitting model to feature number 47, Eubacterium.eligens
2020-10-17 02:46:44 INFO::Fitting model to feature number 48, Eubacterium.hallii
2020-10-17 02:46:44 INFO::Fitting model to feature number 49, Eubacterium.rectale
2020-10-17 02:46:44 INFO::Fitting model to feature number 50, Eubacterium.siraeum
2020-10-17 02:46:44 INFO::Fitting model to feature number 51, Eubacterium.sp.3.1.31
2020-10-17 02:46:45 INFO::Fitting model to feature number 52, Eubacterium.ventriosum
2020-10-17 02:46:45 INFO::Fitting model to feature number 53, Ruminococcus.gnavus
2020-10-17 02:46:45 INFO::Fitting model to feature number 54, Ruminococcus.obeum
2020-10-17 02:46:45 INFO::Fitting model to feature number 55, Ruminococcus.torques
2020-10-17 02:46:45 INFO::Fitting model to feature number 56, Coprococcus.comes
2020-10-17 02:46:45 INFO::Fitting model to feature number 57, Dorea.longicatena
2020-10-17 02:46:46 INFO::Fitting model to feature number 58, Lachnospiraceae.bacterium.1.1.57FAA
2020-10-17 02:46:46 INFO::Fitting model to feature number 59, Lachnospiraceae.bacterium.3.1.46FAA
2020-10-17 02:46:46 INFO::Fitting model to feature number 60, Roseburia.hominis
2020-10-17 02:46:46 INFO::Fitting model to feature number 61, Roseburia.intestinalis
2020-10-17 02:46:46 INFO::Fitting model to feature number 62, Roseburia.inulinivorans
2020-10-17 02:46:46 INFO::Fitting model to feature number 63, Roseburia.unclassified
2020-10-17 02:46:47 INFO::Fitting model to feature number 64, Oscillibacter.unclassified
2020-10-17 02:46:47 INFO::Fitting model to feature number 65, Peptostreptococcaceae.noname.unclassified
2020-10-17 02:46:47 INFO::Fitting model to feature number 66, Faecalibacterium.prausnitzii
2020-10-17 02:46:47 INFO::Fitting model to feature number 67, Ruminococcus.bromii
2020-10-17 02:46:47 INFO::Fitting model to feature number 68, Ruminococcus.callidus
2020-10-17 02:46:48 INFO::Fitting model to feature number 69, Ruminococcus.lactaris
2020-10-17 02:46:48 INFO::Fitting model to feature number 70, Subdoligranulum.unclassified
2020-10-17 02:46:48 INFO::Fitting model to feature number 71, Coprobacillus.unclassified
2020-10-17 02:46:48 INFO::Fitting model to feature number 72, Acidaminococcus.unclassified
2020-10-17 02:46:48 INFO::Fitting model to feature number 73, Dialister.invisus
2020-10-17 02:46:48 INFO::Fitting model to feature number 74, Veillonella.atypica
2020-10-17 02:46:49 INFO::Fitting model to feature number 75, Veillonella.dispar
2020-10-17 02:46:49 INFO::Fitting model to feature number 76, Veillonella.parvula
2020-10-17 02:46:49 INFO::Fitting model to feature number 77, Veillonella.unclassified
2020-10-17 02:46:49 INFO::Fitting model to feature number 78, Burkholderiales.bacterium.1.1.47
2020-10-17 02:46:49 INFO::Fitting model to feature number 79, Parasutterella.excrementihominis
2020-10-17 02:46:49 INFO::Fitting model to feature number 80, Sutterella.wadsworthensis
2020-10-17 02:46:50 INFO::Fitting model to feature number 81, Bilophila.unclassified
2020-10-17 02:46:50 INFO::Fitting model to feature number 82, Escherichia.coli
2020-10-17 02:46:50 INFO::Fitting model to feature number 83, Escherichia.unclassified
2020-10-17 02:46:50 INFO::Fitting model to feature number 84, Klebsiella.pneumoniae
2020-10-17 02:46:50 INFO::Fitting model to feature number 85, Haemophilus.parainfluenzae
2020-10-17 02:46:51 INFO::Fitting model to feature number 86, Akkermansia.muciniphila
2020-10-17 02:46:51 INFO::Fitting model to feature number 87, C2likevirus.unclassified
2020-10-17 02:46:51 INFO::Counting total values for each feature
2020-10-17 02:46:51 INFO::Writing residuals to file output/residuals.rds
2020-10-17 02:46:51 INFO::Writing all results to file (ordered by increasing q-values): output/all_results.tsv
2020-10-17 02:46:51 INFO::Writing the significant results (those which are less than or equal to the threshold of 0.250000 ) to file (ordered by increasing q-values): output/significant_results.tsv
[1] "Creating output folder"
[1] "Creating output figures folder"
2020-10-17 02:46:51 INFO::Writing function arguments to log file
2020-10-17 02:46:51 INFO::Verifying options selected are valid
2020-10-17 02:46:51 INFO::Determining format of input files
2020-10-17 02:46:51 INFO::Input format is data samples as rows and metadata samples as rows
2020-10-17 02:46:51 INFO::Formula for random effects: expr ~ (1 | site) + (1 | subject)
2020-10-17 02:46:51 INFO::Formula for fixed effects: expr ~ diagnosis + dysbiosis + antibiotics + age
2020-10-17 02:46:51 INFO::Running selected normalization method: NONE
2020-10-17 02:46:51 INFO::Filter data based on min abundance and min prevalence
2020-10-17 02:46:51 INFO::Total samples in data: 1595
2020-10-17 02:46:51 INFO::Min samples required with min abundance for a feature not to be filtered: 159.500000
2020-10-17 02:46:51 INFO::Total filtered features: 0
2020-10-17 02:46:51 INFO::Filtered feature names:
2020-10-17 02:46:51 INFO::Bypass z-score application to metadata
2020-10-17 02:46:51 INFO::Running selected transform method: AST
2020-10-17 02:46:51 INFO::Running selected analysis method: LM
2020-10-17 02:46:51 INFO::Fitting model to feature number 1, Bifidobacterium.adolescentis
2020-10-17 02:46:51 INFO::Fitting model to feature number 2, Bifidobacterium.bifidum
2020-10-17 02:46:52 INFO::Fitting model to feature number 3, Bifidobacterium.longum
2020-10-17 02:46:52 INFO::Fitting model to feature number 4, Bifidobacterium.pseudocatenulatum
2020-10-17 02:46:52 INFO::Fitting model to feature number 5, Collinsella.aerofaciens
2020-10-17 02:46:52 INFO::Fitting model to feature number 6, Bacteroides.caccae
2020-10-17 02:46:52 INFO::Fitting model to feature number 7, Bacteroides.cellulosilyticus
2020-10-17 02:46:52 INFO::Fitting model to feature number 8, Bacteroides.dorei
2020-10-17 02:46:53 INFO::Fitting model to feature number 9, Bacteroides.eggerthii
2020-10-17 02:46:53 INFO::Fitting model to feature number 10, Bacteroides.faecis
2020-10-17 02:46:53 INFO::Fitting model to feature number 11, Bacteroides.finegoldii
2020-10-17 02:46:53 INFO::Fitting model to feature number 12, Bacteroides.fragilis
2020-10-17 02:46:54 INFO::Fitting model to feature number 13, Bacteroides.intestinalis
2020-10-17 02:46:54 INFO::Fitting model to feature number 14, Bacteroides.massiliensis
2020-10-17 02:46:54 INFO::Fitting model to feature number 15, Bacteroides.ovatus
2020-10-17 02:46:54 INFO::Fitting model to feature number 16, Bacteroides.salyersiae
2020-10-17 02:46:55 INFO::Fitting model to feature number 17, Bacteroides.stercoris
2020-10-17 02:46:55 INFO::Fitting model to feature number 18, Bacteroides.thetaiotaomicron
2020-10-17 02:46:55 INFO::Fitting model to feature number 19, Bacteroides.uniformis
2020-10-17 02:46:55 INFO::Fitting model to feature number 20, Bacteroides.vulgatus
2020-10-17 02:46:55 INFO::Fitting model to feature number 21, Bacteroides.xylanisolvens
2020-10-17 02:46:56 INFO::Fitting model to feature number 22, Bacteroidales.bacterium.ph8
2020-10-17 02:46:56 INFO::Fitting model to feature number 23, Barnesiella.intestinihominis
2020-10-17 02:46:56 INFO::Fitting model to feature number 24, Coprobacter.fastidiosus
2020-10-17 02:46:56 INFO::Fitting model to feature number 25, Odoribacter.splanchnicus
2020-10-17 02:46:57 INFO::Fitting model to feature number 26, Parabacteroides.distasonis
2020-10-17 02:46:57 INFO::Fitting model to feature number 27, Parabacteroides.goldsteinii
2020-10-17 02:46:57 INFO::Fitting model to feature number 28, Parabacteroides.merdae
2020-10-17 02:46:57 INFO::Fitting model to feature number 29, Parabacteroides.unclassified
2020-10-17 02:46:58 INFO::Fitting model to feature number 30, Paraprevotella.clara
2020-10-17 02:46:58 INFO::Fitting model to feature number 31, Paraprevotella.unclassified
2020-10-17 02:46:58 INFO::Fitting model to feature number 32, Prevotella.copri
2020-10-17 02:46:58 INFO::Fitting model to feature number 33, Alistipes.finegoldii
2020-10-17 02:46:58 INFO::Fitting model to feature number 34, Alistipes.onderdonkii
2020-10-17 02:46:58 INFO::Fitting model to feature number 35, Alistipes.putredinis
2020-10-17 02:46:59 INFO::Fitting model to feature number 36, Alistipes.shahii
2020-10-17 02:46:59 INFO::Fitting model to feature number 37, Alistipes.unclassified
2020-10-17 02:46:59 INFO::Fitting model to feature number 38, Streptococcus.salivarius
2020-10-17 02:46:59 INFO::Fitting model to feature number 39, Clostridium.bolteae
2020-10-17 02:46:59 INFO::Fitting model to feature number 40, Clostridium.citroniae
2020-10-17 02:46:59 INFO::Fitting model to feature number 41, Clostridium.clostridioforme
2020-10-17 02:47:00 INFO::Fitting model to feature number 42, Clostridium.hathewayi
2020-10-17 02:47:00 INFO::Fitting model to feature number 43, Clostridium.leptum
2020-10-17 02:47:00 INFO::Fitting model to feature number 44, Clostridium.nexile
2020-10-17 02:47:00 INFO::Fitting model to feature number 45, Clostridium.symbiosum
2020-10-17 02:47:00 INFO::Fitting model to feature number 46, Flavonifractor.plautii
2020-10-17 02:47:00 INFO::Fitting model to feature number 47, Eubacterium.eligens
2020-10-17 02:47:01 INFO::Fitting model to feature number 48, Eubacterium.hallii
2020-10-17 02:47:01 INFO::Fitting model to feature number 49, Eubacterium.rectale
2020-10-17 02:47:01 INFO::Fitting model to feature number 50, Eubacterium.siraeum
2020-10-17 02:47:01 INFO::Fitting model to feature number 51, Eubacterium.sp.3.1.31
2020-10-17 02:47:01 INFO::Fitting model to feature number 52, Eubacterium.ventriosum
2020-10-17 02:47:02 INFO::Fitting model to feature number 53, Ruminococcus.gnavus
2020-10-17 02:47:02 INFO::Fitting model to feature number 54, Ruminococcus.obeum
2020-10-17 02:47:02 INFO::Fitting model to feature number 55, Ruminococcus.torques
2020-10-17 02:47:02 INFO::Fitting model to feature number 56, Coprococcus.comes
2020-10-17 02:47:02 INFO::Fitting model to feature number 57, Dorea.longicatena
2020-10-17 02:47:03 INFO::Fitting model to feature number 58, Lachnospiraceae.bacterium.1.1.57FAA
2020-10-17 02:47:03 INFO::Fitting model to feature number 59, Lachnospiraceae.bacterium.3.1.46FAA
2020-10-17 02:47:03 INFO::Fitting model to feature number 60, Roseburia.hominis
2020-10-17 02:47:03 INFO::Fitting model to feature number 61, Roseburia.intestinalis
2020-10-17 02:47:04 INFO::Fitting model to feature number 62, Roseburia.inulinivorans
2020-10-17 02:47:04 INFO::Fitting model to feature number 63, Roseburia.unclassified
2020-10-17 02:47:04 INFO::Fitting model to feature number 64, Oscillibacter.unclassified
2020-10-17 02:47:04 INFO::Fitting model to feature number 65, Peptostreptococcaceae.noname.unclassified
2020-10-17 02:47:05 INFO::Fitting model to feature number 66, Faecalibacterium.prausnitzii
2020-10-17 02:47:05 INFO::Fitting model to feature number 67, Ruminococcus.bromii
2020-10-17 02:47:05 INFO::Fitting model to feature number 68, Ruminococcus.callidus
2020-10-17 02:47:05 INFO::Fitting model to feature number 69, Ruminococcus.lactaris
2020-10-17 02:47:06 INFO::Fitting model to feature number 70, Subdoligranulum.unclassified
2020-10-17 02:47:06 INFO::Fitting model to feature number 71, Coprobacillus.unclassified
2020-10-17 02:47:06 INFO::Fitting model to feature number 72, Acidaminococcus.unclassified
2020-10-17 02:47:06 INFO::Fitting model to feature number 73, Dialister.invisus
2020-10-17 02:47:07 INFO::Fitting model to feature number 74, Veillonella.atypica
2020-10-17 02:47:07 INFO::Fitting model to feature number 75, Veillonella.dispar
2020-10-17 02:47:07 INFO::Fitting model to feature number 76, Veillonella.parvula
2020-10-17 02:47:07 INFO::Fitting model to feature number 77, Veillonella.unclassified
2020-10-17 02:47:07 INFO::Fitting model to feature number 78, Burkholderiales.bacterium.1.1.47
2020-10-17 02:47:08 INFO::Fitting model to feature number 79, Parasutterella.excrementihominis
2020-10-17 02:47:08 INFO::Fitting model to feature number 80, Sutterella.wadsworthensis
2020-10-17 02:47:08 INFO::Fitting model to feature number 81, Bilophila.unclassified
2020-10-17 02:47:08 INFO::Fitting model to feature number 82, Escherichia.coli
2020-10-17 02:47:08 INFO::Fitting model to feature number 83, Escherichia.unclassified
2020-10-17 02:47:08 INFO::Fitting model to feature number 84, Klebsiella.pneumoniae
2020-10-17 02:47:09 INFO::Fitting model to feature number 85, Haemophilus.parainfluenzae
2020-10-17 02:47:09 INFO::Fitting model to feature number 86, Akkermansia.muciniphila
2020-10-17 02:47:09 INFO::Fitting model to feature number 87, C2likevirus.unclassified
2020-10-17 02:47:09 INFO::Counting total values for each feature
2020-10-17 02:47:09 INFO::Writing residuals to file output2/residuals.rds
2020-10-17 02:47:09 INFO::Writing all results to file (ordered by increasing q-values): output2/all_results.tsv
2020-10-17 02:47:09 INFO::Writing the significant results (those which are less than or equal to the threshold of 0.250000 ) to file (ordered by increasing q-values): output2/significant_results.tsv
══ testthat results ═══════════════════════════════════════════════════════════
[ OK: 12 | SKIPPED: 0 | WARNINGS: 7 | FAILED: 0 ]
>
>
> proc.time()
user system elapsed
42.587 0.195 42.766