\name{read.spot}
\alias{read.spot}
\title{Read Spot from File}
\description{
Read all file, but only extract the interested columns and create a Spot object.
}
\usage{
read.spot(file.name, cy3, cy5, bg.cy3, bg.cy5, ids, symdesc, header =
FALSE, sep = "\t", is.ifc = FALSE, envir)
}
\arguments{
\item{file.name}{a connection or a character string giving the
name of the file to read where each column represent the spot components.}
\item{cy3}{column that represent Cy3.}
\item{cy5}{column that represent Cy5.}
\item{bg.cy3}{column that represent BgCy3.}
\item{bg.cy5}{column that represent BgCy5.}
\item{ids}{column that represent Id.}
\item{symdesc}{\textbf{optional} identifier besides Id column.}
\item{header}{the logical value of the header input file}
\item{sep}{the separator in the inputfile}
\item{is.ifc}{If is.ifc = TRUE this experiment was done in the
  Unit of Microarray  from  Cellular Phisiology Institute.}
\item{envir}{Environment where are the genArise variables. You don't
  need to specify this argument.}
}
\seealso{
\code{\link{write.spot}}.
}
\keyword{file}