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CHECK report for EDASeq on tokay1

This page was generated on 2018-04-12 13:21:12 -0400 (Thu, 12 Apr 2018).

Package 407/1472HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
EDASeq 2.12.0
Davide Risso
Snapshot Date: 2018-04-11 16:45:18 -0400 (Wed, 11 Apr 2018)
URL: https://git.bioconductor.org/packages/EDASeq
Branch: RELEASE_3_6
Last Commit: ac3b7bf
Last Changed Date: 2017-10-30 12:39:36 -0400 (Mon, 30 Oct 2017)
malbec1 Linux (Ubuntu 16.04.1 LTS) / x86_64  OK  OK  OK UNNEEDED, same version exists in internal repository
tokay1 Windows Server 2012 R2 Standard / x64  OK  OK [ OK ] OK UNNEEDED, same version exists in internal repository
veracruz1 OS X 10.11.6 El Capitan / x86_64  OK  OK  OK  OK UNNEEDED, same version exists in internal repository

Summary

Package: EDASeq
Version: 2.12.0
Command: rm -rf EDASeq.buildbin-libdir EDASeq.Rcheck && mkdir EDASeq.buildbin-libdir EDASeq.Rcheck && C:\Users\biocbuild\bbs-3.6-bioc\R\bin\R.exe CMD INSTALL --build --merge-multiarch --library=EDASeq.buildbin-libdir EDASeq_2.12.0.tar.gz >EDASeq.Rcheck\00install.out 2>&1 && cp EDASeq.Rcheck\00install.out EDASeq-install.out && C:\Users\biocbuild\bbs-3.6-bioc\R\bin\R.exe CMD check --library=EDASeq.buildbin-libdir --install="check:EDASeq-install.out" --force-multiarch --no-vignettes --timings EDASeq_2.12.0.tar.gz
StartedAt: 2018-04-11 23:42:22 -0400 (Wed, 11 Apr 2018)
EndedAt: 2018-04-11 23:48:58 -0400 (Wed, 11 Apr 2018)
EllapsedTime: 396.7 seconds
RetCode: 0
Status:  OK  
CheckDir: EDASeq.Rcheck
Warnings: 0

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   rm -rf EDASeq.buildbin-libdir EDASeq.Rcheck && mkdir EDASeq.buildbin-libdir EDASeq.Rcheck && C:\Users\biocbuild\bbs-3.6-bioc\R\bin\R.exe CMD INSTALL --build --merge-multiarch --library=EDASeq.buildbin-libdir EDASeq_2.12.0.tar.gz >EDASeq.Rcheck\00install.out 2>&1 && cp EDASeq.Rcheck\00install.out EDASeq-install.out  &&  C:\Users\biocbuild\bbs-3.6-bioc\R\bin\R.exe CMD check --library=EDASeq.buildbin-libdir --install="check:EDASeq-install.out" --force-multiarch --no-vignettes --timings EDASeq_2.12.0.tar.gz
###
##############################################################################
##############################################################################


* using log directory 'C:/Users/biocbuild/bbs-3.6-bioc/meat/EDASeq.Rcheck'
* using R version 3.4.4 (2018-03-15)
* using platform: x86_64-w64-mingw32 (64-bit)
* using session charset: ISO8859-1
* using option '--no-vignettes'
* checking for file 'EDASeq/DESCRIPTION' ... OK
* this is package 'EDASeq' version '2.12.0'
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking whether package 'EDASeq' can be installed ... OK
* checking installed package size ... OK
* checking package directory ... OK
* checking 'build' directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking R files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* loading checks for arch 'i386'
** checking whether the package can be loaded ... OK
** checking whether the package can be loaded with stated dependencies ... OK
** checking whether the package can be unloaded cleanly ... OK
** checking whether the namespace can be loaded with stated dependencies ... OK
** checking whether the namespace can be unloaded cleanly ... OK
* loading checks for arch 'x64'
** checking whether the package can be loaded ... OK
** checking whether the package can be loaded with stated dependencies ... OK
** checking whether the package can be unloaded cleanly ... OK
** checking whether the namespace can be loaded with stated dependencies ... OK
** checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... NOTE
Namespace in Imports field not imported from: 'methods'
  All declared Imports should be used.
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... NOTE
.availableOrgPkgs: no visible global function definition for
  'available.packages'
.gcLoess : ff: no visible global function definition for 'quantile'
.gcLoess : ff: no visible global function definition for 'loess'
.gcLoess : ff: no visible global function definition for 'predict'
.gcLoess : ff: no visible global function definition for 'median'
.gcQuant: no visible global function definition for 'quantile'
.gcQuant : f : <anonymous>: no visible global function definition for
  'median'
.gcQuant : f : <anonymous>: no visible global function definition for
  'quantile'
.isAvailable: no visible global function definition for 'biocLite'
newSeqExpressionSet: no visible global function definition for 'new'
MDPlot,SeqExpressionSet-numeric: no visible global function definition
  for 'smoothScatter'
MDPlot,SeqExpressionSet-numeric: no visible global function definition
  for 'lines'
MDPlot,SeqExpressionSet-numeric: no visible global function definition
  for 'lowess'
MDPlot,SeqExpressionSet-numeric: no visible global function definition
  for 'abline'
MDPlot,SeqExpressionSet-numeric: no visible global function definition
  for 'points'
MDPlot,matrix-numeric: no visible global function definition for
  'smoothScatter'
MDPlot,matrix-numeric: no visible global function definition for
  'lines'
MDPlot,matrix-numeric: no visible global function definition for
  'lowess'
MDPlot,matrix-numeric: no visible global function definition for
  'abline'
MDPlot,matrix-numeric: no visible global function definition for
  'points'
barplot,BamFileList: no visible global function definition for
  'countBam'
betweenLaneNormalization,matrix: no visible binding for global variable
  'quantile'
betweenLaneNormalization,matrix: no visible binding for global variable
  'median'
biasBoxplot,numeric-numeric: no visible global function definition for
  'quantile'
biasBoxplot,numeric-numeric: no visible global function definition for
  'abline'
biasPlot,matrix-numeric: no visible global function definition for
  'lowess'
biasPlot,matrix-numeric: no visible global function definition for
  'lines'
boxplot,FastqQuality: no visible global function definition for 'as'
counts<-,SeqExpressionSet-ANY: no visible global function definition
  for 'validObject'
exprs<-,SeqExpressionSet-ANY: no visible global function definition for
  'validObject'
initialize,SeqExpressionSet: no visible global function definition for
  'callNextMethod'
meanVarPlot,SeqExpressionSet: no visible global function definition for
  'quantile'
meanVarPlot,SeqExpressionSet: no visible global function definition for
  'smoothScatter'
meanVarPlot,SeqExpressionSet: no visible global function definition for
  'lines'
meanVarPlot,SeqExpressionSet: no visible global function definition for
  'abline'
meanVarPlot,SeqExpressionSet: no visible global function definition for
  'lowess'
normCounts<-,SeqExpressionSet: no visible global function definition
  for 'validObject'
offst<-,SeqExpressionSet: no visible global function definition for
  'validObject'
plot,BamFileList-FastqFileList: no visible global function definition
  for 'elementMetadata'
plot,BamFileList-FastqFileList: no visible global function definition
  for 'countBam'
plotNtFrequency,BamFile: no visible global function definition for
  'matplot'
plotNtFrequency,BamFile: no visible global function definition for
  'abline'
plotNtFrequency,BamFile: no visible global function definition for
  'legend'
plotNtFrequency,ShortRead: no visible global function definition for
  'matplot'
plotNtFrequency,ShortRead: no visible global function definition for
  'abline'
plotNtFrequency,ShortRead: no visible global function definition for
  'legend'
plotPCA,matrix: no visible global function definition for 'text'
plotPCA,matrix: no visible global function definition for 'pairs'
plotQuality,BamFile: no visible global function definition for
  'rainbow'
plotQuality,BamFile: no visible global function definition for 'path'
plotQuality,BamFile: no visible global function definition for 'narrow'
plotQuality,BamFile: no visible global function definition for 'as'
plotQuality,BamFileList: no visible global function definition for
  'rainbow'
plotQuality,BamFileList : <anonymous>: no visible global function
  definition for 'path'
plotQuality,BamFileList : <anonymous>: no visible global function
  definition for 'narrow'
plotQuality,BamFileList : <anonymous>: no visible global function
  definition for 'as'
plotQuality,BamFileList: no visible global function definition for
  'lines'
plotQuality,FastqFileList: no visible global function definition for
  'rainbow'
plotQuality,FastqFileList : <anonymous>: no visible global function
  definition for 'quality'
plotQuality,FastqFileList : <anonymous>: no visible global function
  definition for 'path'
plotQuality,FastqFileList : <anonymous>: no visible global function
  definition for 'narrow'
plotQuality,FastqFileList : <anonymous>: no visible global function
  definition for 'as'
plotQuality,FastqFileList: no visible global function definition for
  'lines'
plotRLE,matrix: no visible global function definition for 'abline'
updateObject,SeqExpressionSet: no visible global function definition
  for 'callNextMethod'
Undefined global functions or variables:
  abline as available.packages biocLite callNextMethod countBam
  elementMetadata legend lines loess lowess matplot median narrow new
  pairs path points predict quality quantile rainbow smoothScatter text
  validObject
Consider adding
  importFrom("grDevices", "rainbow")
  importFrom("graphics", "abline", "legend", "lines", "matplot", "pairs",
             "points", "smoothScatter", "text")
  importFrom("methods", "as", "callNextMethod", "new", "validObject")
  importFrom("stats", "loess", "lowess", "median", "predict", "quantile")
  importFrom("utils", "available.packages")
to your NAMESPACE file (and ensure that your DESCRIPTION Imports field
contains 'methods').
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking contents of 'data' directory ... OK
* checking data for non-ASCII characters ... OK
* checking data for ASCII and uncompressed saves ... OK
* checking installed files from 'inst/doc' ... OK
* checking files in 'vignettes' ... OK
* checking examples ...
** running examples for arch 'i386' ... OK
Examples with CPU or elapsed time > 5s
                          user system elapsed
getGeneLengthAndGCContent 7.59    0.1   13.53
** running examples for arch 'x64' ... OK
Examples with CPU or elapsed time > 5s
                          user system elapsed
getGeneLengthAndGCContent 3.92   0.03    8.56
* checking for unstated dependencies in 'tests' ... OK
* checking tests ...
** running tests for arch 'i386' ...
  Running 'getLengthAndGC.R'
  Running 'plots.R'
 OK
** running tests for arch 'x64' ...
  Running 'getLengthAndGC.R'
  Running 'plots.R'
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes in 'inst/doc' ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE

Status: 2 NOTEs
See
  'C:/Users/biocbuild/bbs-3.6-bioc/meat/EDASeq.Rcheck/00check.log'
for details.



Installation output

EDASeq.Rcheck/00install.out


install for i386

* installing *source* package 'EDASeq' ...
** R
** data
** inst
** preparing package for lazy loading
** help
*** installing help indices
  converting help for package 'EDASeq'
    finding HTML links ... done
    EDASeq-package                          html  
    finding level-2 HTML links ... done

    MDPlot-methods                          html  
    SeqExpressionSet-class                  html  
    barplot-methods                         html  
    betweenLaneNormalization-methods        html  
    biasBoxplot-methods                     html  
    biasPlot-methods                        html  
    boxplot-methods                         html  
    getGeneLengthAndGCContent               html  
    meanVarPlot-methods                     html  
    newSeqExpressionSet                     html  
    plot-methods                            html  
    plotNtFrequency-methods                 html  
    plotPCA-methods                         html  
    plotQuality-methods                     html  
    plotRLE-methods                         html  
    withinLaneNormalization-methods         html  
    yeastGC                                 html  
    yeastLength                             html  
** building package indices
** installing vignettes
** testing if installed package can be loaded
In R CMD INSTALL

install for x64

* installing *source* package 'EDASeq' ...
** testing if installed package can be loaded
* MD5 sums
packaged installation of 'EDASeq' as EDASeq_2.12.0.zip
* DONE (EDASeq)
In R CMD INSTALL
In R CMD INSTALL

Tests output

EDASeq.Rcheck/tests_i386/getLengthAndGC.Rout


R version 3.4.4 (2018-03-15) -- "Someone to Lean On"
Copyright (C) 2018 The R Foundation for Statistical Computing
Platform: i386-w64-mingw32/i386 (32-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(EDASeq)
Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colMeans, colSums, colnames, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, lengths, mapply, match, mget, order, paste, pmax, pmax.int,
    pmin, pmin.int, rank, rbind, rowMeans, rowSums, rownames, sapply,
    setdiff, sort, table, tapply, union, unique, unsplit, which,
    which.max, which.min

Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: ShortRead
Loading required package: BiocParallel
Loading required package: Biostrings
Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following object is masked from 'package:base':

    expand.grid

Loading required package: IRanges
Loading required package: XVector

Attaching package: 'Biostrings'

The following object is masked from 'package:base':

    strsplit

Loading required package: Rsamtools
Loading required package: GenomeInfoDb
Loading required package: GenomicRanges
Loading required package: GenomicAlignments
Loading required package: SummarizedExperiment
Loading required package: DelayedArray
Loading required package: matrixStats

Attaching package: 'matrixStats'

The following objects are masked from 'package:Biobase':

    anyMissing, rowMedians


Attaching package: 'DelayedArray'

The following objects are masked from 'package:matrixStats':

    colMaxs, colMins, colRanges, rowMaxs, rowMins, rowRanges

The following object is masked from 'package:Biostrings':

    type

The following object is masked from 'package:base':

    apply

> library(yeastRNASeq)
> 
> getGeneLengthAndGCContent(id=c("ENSG00000012048", "ENSG00000139618"), org="hsa", mode = "biomart")
Connecting to BioMart ...
Downloading sequences ...
                length        gc
ENSG00000012048   9187 0.4607598
ENSG00000139618  12273 0.3644586
> 
> proc.time()
   user  system elapsed 
  18.01    0.35   22.89 

EDASeq.Rcheck/tests_x64/getLengthAndGC.Rout


R version 3.4.4 (2018-03-15) -- "Someone to Lean On"
Copyright (C) 2018 The R Foundation for Statistical Computing
Platform: x86_64-w64-mingw32/x64 (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(EDASeq)
Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colMeans, colSums, colnames, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, lengths, mapply, match, mget, order, paste, pmax, pmax.int,
    pmin, pmin.int, rank, rbind, rowMeans, rowSums, rownames, sapply,
    setdiff, sort, table, tapply, union, unique, unsplit, which,
    which.max, which.min

Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: ShortRead
Loading required package: BiocParallel
Loading required package: Biostrings
Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following object is masked from 'package:base':

    expand.grid

Loading required package: IRanges
Loading required package: XVector

Attaching package: 'Biostrings'

The following object is masked from 'package:base':

    strsplit

Loading required package: Rsamtools
Loading required package: GenomeInfoDb
Loading required package: GenomicRanges
Loading required package: GenomicAlignments
Loading required package: SummarizedExperiment
Loading required package: DelayedArray
Loading required package: matrixStats

Attaching package: 'matrixStats'

The following objects are masked from 'package:Biobase':

    anyMissing, rowMedians


Attaching package: 'DelayedArray'

The following objects are masked from 'package:matrixStats':

    colMaxs, colMins, colRanges, rowMaxs, rowMins, rowRanges

The following object is masked from 'package:Biostrings':

    type

The following object is masked from 'package:base':

    apply

> library(yeastRNASeq)
> 
> getGeneLengthAndGCContent(id=c("ENSG00000012048", "ENSG00000139618"), org="hsa", mode = "biomart")
Connecting to BioMart ...
Downloading sequences ...
                length        gc
ENSG00000012048   9187 0.4607598
ENSG00000139618  12273 0.3644586
> 
> proc.time()
   user  system elapsed 
  17.60    0.20   22.59 

EDASeq.Rcheck/tests_i386/plots.Rout


R version 3.4.4 (2018-03-15) -- "Someone to Lean On"
Copyright (C) 2018 The R Foundation for Statistical Computing
Platform: i386-w64-mingw32/i386 (32-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(EDASeq)
Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colMeans, colSums, colnames, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, lengths, mapply, match, mget, order, paste, pmax, pmax.int,
    pmin, pmin.int, rank, rbind, rowMeans, rowSums, rownames, sapply,
    setdiff, sort, table, tapply, union, unique, unsplit, which,
    which.max, which.min

Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: ShortRead
Loading required package: BiocParallel
Loading required package: Biostrings
Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

The following object is masked from 'package:base':

    expand.grid

Loading required package: IRanges
Loading required package: XVector

Attaching package: 'Biostrings'

The following object is masked from 'package:base':

    strsplit

Loading required package: Rsamtools
Loading required package: GenomeInfoDb
Loading required package: GenomicRanges
Loading required package: GenomicAlignments
Loading required package: SummarizedExperiment
Loading required package: DelayedArray
Loading required package: matrixStats

Attaching package: 'matrixStats'

The following objects are masked from 'package:Biobase':

    anyMissing, rowMedians


Attaching package: 'DelayedArray'

The following objects are masked from 'package:matrixStats':

    colMaxs, colMins, colRanges, rowMaxs, rowMins, rowRanges

The following object is masked from 'package:Biostrings':

    type

The following object is masked from 'package:base':

    apply

> 
> mat <- matrix(data=rpois(1000, lambda=10), ncol=10)
> rownames(mat) <- paste("gene", 1:nrow(mat), sep="")
> colnames(mat) <- paste("sample", 1:ncol(mat), sep="")
> 
> es <- newSeqExpressionSet(mat)
> 
> ## PCA
> ks <- 2:5
> 
> ## matrix
> lapply(ks, function(k) plotPCA(mat, k=k))
[[1]]
NULL

[[2]]
NULL

[[3]]
NULL

[[4]]
NULL

> lapply(ks, function(k) plotPCA(mat, k=k, labels=FALSE))
[[1]]
NULL

[[2]]
NULL

[[3]]
NULL

[[4]]
NULL

> lapply(ks, function(k) plotPCA(mat, k=k))
[[1]]
NULL

[[2]]
NULL

[[3]]
NULL

[[4]]
NULL

> lapply(ks, function(k) plotPCA(mat, k=k, labels=FALSE, pch=20, col=1:2))
[[1]]
NULL

[[2]]
NULL

[[3]]
NULL

[[4]]
NULL

> 
> ## expressionset
> lapply(ks, function(k) plotPCA(es, k=k))
[[1]]
NULL

[[2]]
NULL

[[3]]
NULL

[[4]]
NULL

> lapply(ks, function(k) plotPCA(es, k=k, labels=FALSE))
[[1]]
NULL

[[2]]
NULL

[[3]]
NULL

[[4]]
NULL

> lapply(ks, function(k) plotPCA(es, k=k, labels=FALSE, pch=20, col=1:2))
[[1]]
NULL

[[2]]
NULL

[[3]]
NULL

[[4]]
NULL

> 
> ## RLE
> 
> ## matrix
> rle <- plotRLE(mat)
> stopifnot(all(dim(mat)==dim(rle)))
> 
> ## expressionset
> rle <- plotRLE(es)
> stopifnot(all(dim(mat)==dim(rle)))
> stopifnot(all(mat==counts(es)))
> 
> proc.time()
   user  system elapsed 
   9.54    0.35    9.89 

EDASeq.Rcheck/tests_x64/plots.Rout


R version 3.4.4 (2018-03-15) -- "Someone to Lean On"
Copyright (C) 2018 The R Foundation for Statistical Computing
Platform: x86_64-w64-mingw32/x64 (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(EDASeq)
Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colMeans, colSums, colnames, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, lengths, mapply, match, mget, order, paste, pmax, pmax.int,
    pmin, pmin.int, rank, rbind, rowMeans, rowSums, rownames, sapply,
    setdiff, sort, table, tapply, union, unique, unsplit, which,
    which.max, which.min

Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: ShortRead
Loading required package: BiocParallel
Loading required package: Biostrings
Loading required package: S4Vectors
Loading required package: stats4

Attaching package: 'S4Vectors'

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Loading required package: Rsamtools
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    apply

> 
> mat <- matrix(data=rpois(1000, lambda=10), ncol=10)
> rownames(mat) <- paste("gene", 1:nrow(mat), sep="")
> colnames(mat) <- paste("sample", 1:ncol(mat), sep="")
> 
> es <- newSeqExpressionSet(mat)
> 
> ## PCA
> ks <- 2:5
> 
> ## matrix
> lapply(ks, function(k) plotPCA(mat, k=k))
[[1]]
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[[2]]
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[[3]]
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[[4]]
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> lapply(ks, function(k) plotPCA(mat, k=k, labels=FALSE))
[[1]]
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[[2]]
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[[3]]
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[[4]]
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> lapply(ks, function(k) plotPCA(mat, k=k))
[[1]]
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[[2]]
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[[3]]
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[[4]]
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> lapply(ks, function(k) plotPCA(mat, k=k, labels=FALSE, pch=20, col=1:2))
[[1]]
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[[2]]
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[[3]]
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> 
> ## expressionset
> lapply(ks, function(k) plotPCA(es, k=k))
[[1]]
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[[2]]
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[[3]]
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[[4]]
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> lapply(ks, function(k) plotPCA(es, k=k, labels=FALSE))
[[1]]
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> lapply(ks, function(k) plotPCA(es, k=k, labels=FALSE, pch=20, col=1:2))
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> 
> ## RLE
> 
> ## matrix
> rle <- plotRLE(mat)
> stopifnot(all(dim(mat)==dim(rle)))
> 
> ## expressionset
> rle <- plotRLE(es)
> stopifnot(all(dim(mat)==dim(rle)))
> stopifnot(all(mat==counts(es)))
> 
> proc.time()
   user  system elapsed 
  10.39    0.20   10.57 

Example timings

EDASeq.Rcheck/examples_i386/EDASeq-Ex.timings

nameusersystemelapsed
MDPlot-methods0.310.030.44
SeqExpressionSet-class0.160.000.15
betweenLaneNormalization-methods0.110.000.11
biasBoxplot-methods0.090.020.11
biasPlot-methods0.180.030.20
getGeneLengthAndGCContent 7.59 0.1013.53
newSeqExpressionSet0.060.000.07
plotPCA-methods0.660.060.71
plotRLE-methods0.440.010.46
withinLaneNormalization-methods0.180.030.22

EDASeq.Rcheck/examples_x64/EDASeq-Ex.timings

nameusersystemelapsed
MDPlot-methods0.760.020.78
SeqExpressionSet-class0.190.000.19
betweenLaneNormalization-methods0.080.030.10
biasBoxplot-methods0.070.040.11
biasPlot-methods0.190.010.21
getGeneLengthAndGCContent3.920.038.56
newSeqExpressionSet0.080.000.08
plotPCA-methods1.300.081.37
plotRLE-methods0.500.030.53
withinLaneNormalization-methods0.200.030.24