############################################################################## ############################################################################## ### ### Running command: ### ### /home/biocbuild/bbs-3.17-bioc/R/bin/R CMD check --install=check:EnrichmentBrowser.install-out.txt --library=/home/biocbuild/bbs-3.17-bioc/R/site-library --timings EnrichmentBrowser_2.29.2.tar.gz ### ############################################################################## ############################################################################## * using log directory ‘/home/biocbuild/bbs-3.17-bioc/meat/EnrichmentBrowser.Rcheck’ * using R version 4.3.0 alpha (2023-04-03 r84154) * using platform: x86_64-pc-linux-gnu (64-bit) * R was compiled by gcc (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0 GNU Fortran (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0 * running under: Ubuntu 22.04.2 LTS * using session charset: UTF-8 * checking for file ‘EnrichmentBrowser/DESCRIPTION’ ... OK * this is package ‘EnrichmentBrowser’ version ‘2.29.2’ * package encoding: UTF-8 * checking package namespace information ... OK * checking package dependencies ... OK * checking if this is a source package ... OK * checking if there is a namespace ... OK * checking for hidden files and directories ... OK * checking for portable file names ... OK * checking for sufficient/correct file permissions ... OK * checking whether package ‘EnrichmentBrowser’ can be installed ... OK * checking installed package size ... OK * checking package directory ... OK * checking ‘build’ directory ... OK * checking DESCRIPTION meta-information ... OK * checking top-level files ... OK * checking for left-over files ... OK * checking index information ... OK * checking package subdirectories ... OK * checking R files for non-ASCII characters ... OK * checking R files for syntax errors ... OK * checking whether the package can be loaded ... OK * checking whether the package can be loaded with stated dependencies ... OK * checking whether the package can be unloaded cleanly ... OK * checking whether the namespace can be loaded with stated dependencies ... OK * checking whether the namespace can be unloaded cleanly ... OK * checking loading without being on the library search path ... OK * checking startup messages can be suppressed ... OK * checking dependencies in R code ... NOTE Unexported object imported by a ':::' call: 'pathview:::parseKGML2Graph2' See the note in ?`:::` about the use of this operator. * checking S3 generic/method consistency ... OK * checking replacement functions ... OK * checking foreign function calls ... OK * checking R code for possible problems ... NOTE .getGOFromBiomart: no visible binding for global variable ‘go_linkage_type’ Undefined global functions or variables: go_linkage_type * checking Rd files ... OK * checking Rd metadata ... OK * checking Rd cross-references ... OK * checking for missing documentation entries ... OK * checking for code/documentation mismatches ... OK * checking Rd \usage sections ... OK * checking Rd contents ... OK * checking for unstated dependencies in examples ... OK * checking files in ‘vignettes’ ... OK * checking examples ... ERROR Running examples in ‘EnrichmentBrowser-Ex.R’ failed The error most likely occurred in: > base::assign(".ptime", proc.time(), pos = "CheckExEnv") > ### Name: import > ### Title: Import results from differential expression (DE) analysis > ### Aliases: import > > ### ** Examples > > > # Setup > ## i) Generate count data > nsamples <- 4 > ngenes <- 1000 > dispers <- 1 / rchisq(ngenes, df = 10) > rdesign <- model.matrix(~factor(rep(c(1, 2), each = 2))) > > counts <- rnbinom(ngenes * nsamples, mu = 20, size = 1 / dispers) > counts <- matrix(counts, nrow = ngenes, ncol = nsamples) > > ## ii) Generate intensity data > sd <- 0.3 * sqrt(4 / rchisq(100, df = 4)) > intens <- matrix(rnorm(100 * 6, sd = sd), nrow = 100, ncol = 6) > rownames(intens) <- paste0("Gene", 1:100) > intens[1:2, 4:6] <- intens[1:2, 4:6] + 2 > mdesign <- cbind(Grp1 = 1, Grp2vs1 = rep(c(0,1), each = 3)) > > # (1) import from edgeR (RNA-seq count data) > # (1a) create the expression data object > library(edgeR) Loading required package: limma Attaching package: 'limma' The following object is masked from 'package:BiocGenerics': plotMA > d <- DGEList(counts) > d <- calcNormFactors(d) > d <- estimateDisp(d, rdesign) > > # (1b) obtain differential expression results > fit <- glmQLFit(d, rdesign) > qlf <- glmQLFTest(fit) > res <- topTags(qlf, n = nrow(d), sort.by = "none") > > # (1c) import > se <- import(d, res) > > # (2) import from DESeq2 (RNA-seq count data) > # (2a) create the expression data object > library(DESeq2) > condition <- factor(rep(c("A", "B"), each = 2)) > dds <- DESeqDataSetFromMatrix(counts, + colData = DataFrame(condition = condition), + design = ~ condition) converting counts to integer mode > > # (2b) obtain differential expression results > dds <- DESeq(dds) estimating size factors estimating dispersions gene-wise dispersion estimates mean-dispersion relationship final dispersion estimates fitting model and testing > res <- results(dds) > > # (2c) import > se <- import(dds, res) > > # (3) import from voom/limma (RNA-seq count data) > # (3a) create the expression data object > library(limma) > keep <- filterByExpr(counts, rdesign) > el <- voom(counts[keep,], rdesign) > > # (3b) obtain differential expression results > fit <- lmFit(el, rdesign) > fit <- eBayes(fit, robust = TRUE) Error in fitFDistRobustly(var, df1 = df, covariate = covariate, winsor.tail.p = winsor.tail.p) : statmod package required but is not installed Calls: eBayes -> .ebayes -> squeezeVar -> fitFDistRobustly Execution halted * checking for unstated dependencies in vignettes ... OK * checking package vignettes in ‘inst/doc’ ... OK * checking running R code from vignettes ... ‘EnrichmentBrowser.Rnw’ using ‘UTF-8’... OK NONE * checking re-building of vignette outputs ... ERROR Error(s) in re-building vignettes: ... --- re-building ‘EnrichmentBrowser.Rnw’ using knitr Quitting from lines 371-373 (EnrichmentBrowser.Rnw) Error: processing vignette 'EnrichmentBrowser.Rnw' failed with diagnostics: statmod package required but is not installed --- failed re-building ‘EnrichmentBrowser.Rnw’ SUMMARY: processing the following file failed: ‘EnrichmentBrowser.Rnw’ Error: Vignette re-building failed. Execution halted * checking PDF version of manual ... OK * DONE Status: 2 ERRORs, 2 NOTEs See ‘/home/biocbuild/bbs-3.17-bioc/meat/EnrichmentBrowser.Rcheck/00check.log’ for details.