############################################################################## ############################################################################## ### ### Running command: ### ### /home/biocbuild/bbs-3.17-bioc/R/bin/R CMD check --install=check:EGSEA.install-out.txt --library=/home/biocbuild/bbs-3.17-bioc/R/site-library --timings EGSEA_1.27.0.tar.gz ### ############################################################################## ############################################################################## * using log directory ‘/home/biocbuild/bbs-3.17-bioc/meat/EGSEA.Rcheck’ * using R version 4.3.0 alpha (2023-04-03 r84154) * using platform: x86_64-pc-linux-gnu (64-bit) * R was compiled by gcc (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0 GNU Fortran (Ubuntu 11.3.0-1ubuntu1~22.04) 11.3.0 * running under: Ubuntu 22.04.2 LTS * using session charset: UTF-8 * checking for file ‘EGSEA/DESCRIPTION’ ... OK * this is package ‘EGSEA’ version ‘1.27.0’ * checking package namespace information ... OK * checking package dependencies ... OK * checking if this is a source package ... OK * checking if there is a namespace ... OK * checking for hidden files and directories ... OK * checking for portable file names ... OK * checking for sufficient/correct file permissions ... OK * checking whether package ‘EGSEA’ can be installed ... OK * checking installed package size ... OK * checking package directory ... OK * checking ‘build’ directory ... OK * checking DESCRIPTION meta-information ... OK * checking top-level files ... OK * checking for left-over files ... OK * checking index information ... OK * checking package subdirectories ... OK * checking R files for non-ASCII characters ... OK * checking R files for syntax errors ... OK * checking whether the package can be loaded ... OK * checking whether the package can be loaded with stated dependencies ... OK * checking whether the package can be unloaded cleanly ... OK * checking whether the namespace can be loaded with stated dependencies ... OK * checking whether the namespace can be unloaded cleanly ... OK * checking loading without being on the library search path ... OK * checking dependencies in R code ... OK * checking S3 generic/method consistency ... OK * checking replacement functions ... OK * checking foreign function calls ... OK * checking R code for possible problems ... NOTE buildGeneSetDBIdx: no visible binding for global variable ‘GOTERM’ buildMSigDBIdx: no visible binding for global variable ‘msigdb’ generateSummaryPlots: no visible binding for global variable ‘x.data’ generateSummaryPlots: no visible binding for global variable ‘y.data’ generateSummaryPlots: no visible binding for global variable ‘gsSize’ generateSummaryPlots: no visible binding for global variable ‘id’ generateSummaryPlots: no visible binding for global variable ‘sig’ loadKeggData: no visible binding for global variable ‘kegg.pathways’ Undefined global functions or variables: GOTERM gsSize id kegg.pathways msigdb sig x.data y.data * checking Rd files ... OK * checking Rd metadata ... OK * checking Rd cross-references ... OK * checking for missing documentation entries ... WARNING Undocumented data sets: ‘arraydata’ All user-level objects in a package should have documentation entries. See chapter ‘Writing R documentation files’ in the ‘Writing R Extensions’ manual. * checking for code/documentation mismatches ... OK * checking Rd \usage sections ... OK * checking Rd contents ... OK * checking for unstated dependencies in examples ... WARNING 'library' or 'require' call not declared from: ‘Glimma’ * checking contents of ‘data’ directory ... OK * checking data for non-ASCII characters ... OK * checking data for ASCII and uncompressed saves ... OK * checking files in ‘vignettes’ ... OK * checking examples ... ERROR Running examples in ‘EGSEA-Ex.R’ failed The error most likely occurred in: > base::assign(".ptime", proc.time(), pos = "CheckExEnv") > ### Name: egsea > ### Title: Core functions to perform ensemble of gene set enrichment > ### analysis (EGSEA) > ### Aliases: egsea egsea,egsea-main egsea.cnt egsea.cnt,egsea-main > ### egsea.ora egsea.ora,egsea-main egsea.cnt egsea.ma > ### egsea.cnt,egsea-main > > ### ** Examples > > # Example of egsea > library(EGSEAdata) > data(il13.data) > v = il13.data$voom > contrasts = il13.data$contra > gs.annots = buildIdx(entrezIDs=rownames(v$E), species="human", + msigdb.gsets="none", + kegg.updated=FALSE, kegg.exclude = c("Metabolism")) [1] "Building KEGG pathways annotation object ... " > # set report = TRUE to generate the EGSEA interactive report > gsa = egsea(voom.results=v, contrasts=contrasts, gs.annots=gs.annots, + symbolsMap=v$genes, baseGSEAs=egsea.base()[-c(2,5,6,9,12)], + display.top = 5, sort.by="avg.rank", + report.dir="./il13-egsea-report", + num.threads = 2, report = FALSE) EGSEA analysis has started ##------ Tue Apr 11 20:12:02 2023 ------## Log fold changes are estimated using limma package ... limma DE analysis is carried out ... EGSEA is running on the provided data and kegg collection ...safe*....zscore*..globaltest*camera*..gage*...gsva*..ora* ##------ Tue Apr 11 20:12:09 2023 ------## EGSEA analysis took 7.64699999999999 seconds. EGSEA analysis has completed > topSets(gsa) Extracting the top gene sets of the collection KEGG Pathways for the contrast X24IL13-X24 Sorted by avg.rank [1] "Intestinal immune network for IgA production" [2] "Asthma" [3] "Amoebiasis" [4] "Hematopoietic cell lineage" [5] "HTLV-I infection" [6] "Viral myocarditis" [7] "Malaria" [8] "Endocrine and other factor-regulated calcium reabsorption" [9] "Legionellosis" [10] "Proteoglycans in cancer" > > # Example of egsea.cnt > library(EGSEAdata) > data(il13.data.cnt) > cnt = il13.data.cnt$counts > group = il13.data.cnt$group > design = il13.data.cnt$design > contrasts = il13.data.cnt$contra > genes = il13.data.cnt$genes > gs.annots = buildIdx(entrezIDs=rownames(cnt), species="human", + msigdb.gsets="none", + kegg.updated=FALSE, kegg.exclude = c("Metabolism")) [1] "Building KEGG pathways annotation object ... " > # set report = TRUE to generate the EGSEA interactive report > gsa = egsea.cnt(counts=cnt, group=group, design=design, contrasts=contrasts, + gs.annots=gs.annots, + symbolsMap=genes, baseGSEAs=egsea.base()[-c(2,5,6,9,12)], + display.top = 5, + sort.by="avg.rank", + report.dir="./il13-egsea-cnt-report", + num.threads = 2, report = FALSE) EGSEA analysis has started ##------ Tue Apr 11 20:12:10 2023 ------## Log fold changes are estimated using limma package ... limma DE analysis is carried out ... EGSEA is running on the provided data and kegg collection ...safe*....zscore*..globaltest*camera*..gage*...gsva*..ora* ##------ Tue Apr 11 20:12:18 2023 ------## EGSEA analysis took 7.67000000000002 seconds. EGSEA analysis has completed > topSets(gsa) Extracting the top gene sets of the collection KEGG Pathways for the contrast X24IL13-X24 Sorted by avg.rank [1] "Intestinal immune network for IgA production" [2] "Asthma" [3] "Amoebiasis" [4] "Malaria" [5] "Hematopoietic cell lineage" [6] "HTLV-I infection" [7] "Viral myocarditis" [8] "Endocrine and other factor-regulated calcium reabsorption" [9] "Legionellosis" [10] "Prion diseases" > > # Example of egsea.ora > library(EGSEAdata) > data(il13.data) > voom.results = il13.data$voom > contrast = il13.data$contra > library(limma) Attaching package: ‘limma’ The following object is masked from ‘package:BiocGenerics’: plotMA > vfit = lmFit(voom.results, voom.results$design) > vfit = contrasts.fit(vfit, contrast) > vfit = eBayes(vfit) > top.Table = topTable(vfit, coef=1, number=Inf, p.value=0.05, lfc=1) > deGenes = as.character(top.Table$FeatureID) > logFC = top.Table$logFC > names(logFC) = deGenes > gs.annots = buildIdx(entrezIDs=deGenes, species="human", + msigdb.gsets="none", + kegg.updated=FALSE, kegg.exclude = c("Metabolism")) [1] "Building KEGG pathways annotation object ... " > # set report = TRUE to generate the EGSEA interactive report > gsa = egsea.ora(geneIDs=deGenes, universe= + as.character(voom.results$genes[,1]), + logFC =logFC, title="X24IL13-X24", + gs.annots=gs.annots, + symbolsMap=top.Table[, c(1,2)], display.top = 5, + report.dir="./il13-egsea-ora-report", num.threads = 2, + report = FALSE) EGSEA analysis has started ##------ Tue Apr 11 20:12:20 2023 ------## EGSEA is running on the provided data and kegg collection .ora* ##------ Tue Apr 11 20:12:20 2023 ------## EGSEA analysis took 0.144000000000005 seconds. EGSEA analysis has completed > topSets(gsa) Extracting the top gene sets of the collection KEGG Pathways for the contrast X24IL13X24 Sorted by p.adj [1] "Hematopoietic cell lineage" [2] "Staphylococcus aureus infection" [3] "Cytokine-cytokine receptor interaction" [4] "Phagosome" [5] "Tuberculosis" [6] "Leishmaniasis" [7] "Cell adhesion molecules (CAMs)" [8] "Rheumatoid arthritis" [9] "Asthma" [10] "Type I diabetes mellitus" > > # Example of egsea.ma > library(Glimma) Error in library(Glimma) : there is no package called ‘Glimma’ Execution halted * checking for unstated dependencies in ‘tests’ ... OK * checking tests ... Running ‘testthat.R’ OK * checking for unstated dependencies in vignettes ... OK * checking package vignettes in ‘inst/doc’ ... OK * checking running R code from vignettes ... ‘EGSEA.Rnw’... OK NONE * checking re-building of vignette outputs ... OK * checking PDF version of manual ... OK * DONE Status: 1 ERROR, 2 WARNINGs, 1 NOTE See ‘/home/biocbuild/bbs-3.17-bioc/meat/EGSEA.Rcheck/00check.log’ for details.