fig:qc-plot-pancreas fig:qc-plot-pancreas-better fig:discardplot416b fig:discardplotpbmc fig:zeisel-demo-snap25 fig:cellbench-lognorm-fail fig:cellbench-lognorm-downsample fig:trend-plot-seger-noweight fig:cv2-pbmc fig:elbow fig:cluster-pc-choice fig:zeisel-parallel-pc-choice fig:corral-sort fig:snifter-scater fig:snifter-embedding fig:densne fig:pbmc-silhouette fig:pbmc-box-purities fig:rmsd-pbmc fig:rmsd-pbmc-lib fig:cluster-mod fig:cluster-graph fig:walktrap-v-louvain-prop fig:walktrap-v-louvain-jaccard fig:pbmc-graph-linked fig:walktrap-res-clustree fig:pbmc-rand-breakdown fig:bootstrap-matrix fig:graph-parameter-sweep fig:simulated-outliers-auc-cohen fig:simulated-cap-auc-cohen fig:viol-gcg-lawlor fig:pval-dist fig:rankplot fig:ambientpvalhist fig:qc-mito-pbmc fig:ambient-removal-igkc fig:ambient-removal-lyz fig:barcode-rank-mix-genes fig:barcode-rank-mix-hto fig:hto-total-comp fig:hto-1to2-hist fig:hto-ambient fig:hto-ambient2 fig:hto-mix-tsne fig:mammary-swapped-barcode-rank fig:mammary-swapped-barcode-rank-after fig:heatclust fig:markerexprs fig:denstsne fig:densclust fig:scdblfinder-tsne fig:hash-barcode-rank fig:hto-2to3-hash fig:tsne-hash fig:doublet-prop-hash-dist fig:heat-cyclin fig:heat-cyclin-grun fig:dist-lef1 fig:phaseplot416b fig:cell-cycle-regression fig:cell-cycle-regression2 fig:cell-cycle-regression3 fig:leng-nocycle fig:discard-416b fig:cell-cycle-contrastive fig:tscan-nest-tsne fig:tscan-nest-pseudo fig:tscan-nest-omega fig:tscan-nest-mnn fig:traj-princurve-tsne-nest fig:traj-princurve-clustered-nest fig:traj-princurve-omag-nest fig:nest-1-simple-down fig:nest-1-simple-up fig:nest-1-simple-up-heat fig:nest-pseudo-reordered fig:nest-3-versus fig:entropy-nest fig:tsne-hermann-velocity fig:tscan-sperm-velocity fig:tcell-pseudotime fig:nuclei-qc fig:nuclei-tsne fig:nuclei-tsne-merged fig:nuclei-contamination fig:top-prop-adt-dist fig:cite-detected-ab-hist fig:cite-total-con-hist fig:pbmc-adt-ambient-profile fig:comp-bias-norm fig:control-bias-norm fig:tsne-tags fig:heat-tags fig:subcluster-stats fig:gzmh-cd8-t fig:subcluster-tag-dist fig:rescaled-tsne-adt fig:combined-umap fig:correlation-cd127-cluster fig:correlation-pd1-blocked fig:iSEE-default fig:iSEE-landing fig:iSEE-qc fig:iSEE-anno fig:iSEE-hvg fig:iSEE-rowcol fig:iSEE-tour quality-control-redux overview the-isoutlier-function outlier-assumptions qc-batch qc-discard-cell-types more-norm overview-1 scaling-and-the-pseudo-count downsampling-instead-of-scaling comments-on-other-transformations normalization-versus-batch-correction more-hvgs overview-2 fine-tuning-the-fitted-trend handling-covariates-with-linear-models using-the-coefficient-of-variation more-hvg-selection-strategies feature-selection-positive based-on-significance apriori-hvgs dimensionality-reduction-redux overview-3 more-choices-for-the-number-of-pcs using-the-elbow-point using-the-technical-noise based-on-population-structure using-random-matrix-theory count-based-dimensionality-reduction more-visualization-methods fast-interpolation-based-t-sne density-preserving-t-sne-and-umap clustering-redux motivation quantifying-clustering-behavior motivation-1 silhouette-width cluster-purity within-cluster-sum-of-squares using-graph-modularity comparing-different-clusterings motivation-2 identifying-corresponding-clusters visualizing-differences adjusted-rand-index cluster-bootstrapping clustering-parameter-sweeps agglomerating-graph-communities marker-detection-redux motivation-3 properties-of-each-effect-size using-custom-de-methods p-value-invalidity from-data-snooping false-replicates further-comments droplet-processing motivation-4 qc-droplets background testing-for-empty-droplets relationship-with-other-qc-metrics removing-ambient-contamination cell-hashing background-1 cell-calling-options demultiplexing-on-hto-abundance further-comments-1 removing-swapped-molecules doublet-detection overview-4 doublet-detection-with-clusters doublet-simulation computing-doublet-densities doublet-classification further-comments-2 doublet-detection-in-multiplexed-experiments background-2 identifying-inter-sample-doublets guilt-by-association-for-unmarked-doublets further-comments-3 cell-cycle-assignment motivation-5 using-the-cyclins using-reference-profiles using-the-cyclone-classifier removing-cell-cycle-effects comments with-linear-regression-and-friends removing-cell-cycle-related-genes using-contrastive-pca trajectory-analysis overview-5 obtaining-pseudotime-orderings overview-6 cluster-based-minimum-spanning-tree basic-steps tweaking-the-mst further-comments-4 principal-curves characterizing-trajectories overview-7 changes-along-a-trajectory changes-between-paths further-comments-5 finding-the-root overview-8 entropy-based-methods rna-velocity real-timepoints single-nuclei-rna-seq-processing introduction quality-control-for-stripped-nuclei comments-on-downstream-analyses nuclei-ambient-tricks integrating-with-protein-abundance motivation-6 setting-up-the-data quality-control issues-with-rna-based-metrics applying-custom-qc-filters other-comments normalization overview-9 library-size-normalization cite-seq-median-norm control-based-normalization computing-log-normalized-values comments-on-downstream-analyses-1 feature-selection clustering-and-interpretation integration-with-gene-expression-data by-subclustering by-intersecting-clusters with-rescaled-expression-matrices with-multi-metric-umap finding-correlations-between-features interactive-sharing motivation-7 interactive-quickstart isee-examples quality-control-1 annotation-of-cell-populations querying-features-of-interest reproducible-visualizations dissemination additional-resources dealing-with-big-data motivation-8 fast-approximations nearest-neighbor-searching big-data-svd parallelization out-of-memory-representations