CATALYST

This package is for version 3.17 of Bioconductor; for the stable, up-to-date release version, see CATALYST.

Cytometry dATa anALYSis Tools


Bioconductor version: 3.17

Mass cytometry (CyTOF) uses heavy metal isotopes rather than fluorescent tags as reporters to label antibodies, thereby substantially decreasing spectral overlap and allowing for examination of over 50 parameters at the single cell level. While spectral overlap is significantly less pronounced in CyTOF than flow cytometry, spillover due to detection sensitivity, isotopic impurities, and oxide formation can impede data interpretability. We designed CATALYST (Cytometry dATa anALYSis Tools) to provide a pipeline for preprocessing of cytometry data, including i) normalization using bead standards, ii) single-cell deconvolution, and iii) bead-based compensation.

Author: Helena L. Crowell [aut, cre] , Vito R.T. Zanotelli [aut], Stéphane Chevrier [aut, dtc], Mark D. Robinson [aut, fnd], Bernd Bodenmiller [fnd]

Maintainer: Helena L. Crowell <helena.crowell at uzh.ch>

Citation (from within R, enter citation("CATALYST")):

Installation

To install this package, start R (version "4.3") and enter:


if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("CATALYST")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("CATALYST")
1. Preprocessing HTML R Script
2. Differential discovery HTML R Script
Reference Manual PDF
NEWS Text

Details

biocViews Clustering, DataImport, DifferentialExpression, ExperimentalDesign, FlowCytometry, ImmunoOncology, MassSpectrometry, Normalization, Preprocessing, SingleCell, Software, StatisticalMethod, Visualization
Version 1.24.0
In Bioconductor since BioC 3.5 (R-3.4) (7 years)
License GPL (>=2)
Depends R (>= 4.2), SingleCellExperiment
Imports circlize, ComplexHeatmap, ConsensusClusterPlus, cowplot, data.table, dplyr, drc, flowCore, FlowSOM, ggplot2, ggrepel, ggridges, graphics, grDevices, grid, gridExtra, magrittr, Matrix, matrixStats, methods, nnls, purrr, RColorBrewer, reshape2, Rtsne, SummarizedExperiment, S4Vectors, scales, scater, stats
System Requirements
URL https://github.com/HelenaLC/CATALYST
Bug Reports https://github.com/HelenaLC/CATALYST/issues
See More
Suggests BiocStyle, diffcyt, flowWorkspace, ggcyto, knitr, openCyto, rmarkdown, testthat, uwot
Linking To
Enhances
Depends On Me cytofWorkflow
Imports Me cytofQC
Suggests Me diffcyt, imcRtools, treekoR
Links To Me
Build Report Build Report

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package CATALYST_1.24.0.tar.gz
Windows Binary CATALYST_1.24.0.zip (64-bit only)
macOS Binary (x86_64) CATALYST_1.24.0.tgz
macOS Binary (arm64)
Source Repository git clone https://git.bioconductor.org/packages/CATALYST
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/CATALYST
Bioc Package Browser https://code.bioconductor.org/browse/CATALYST/
Package Short Url https://bioconductor.org/packages/CATALYST/
Package Downloads Report Download Stats
Old Source Packages for BioC 3.17 Source Archive